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Leadgene Leadgene
ZIKV E Protein, His Tag, E. coli
Catalog Number

LDG001PVE

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  • Specifications
    • Species of Origin

      Zika virus

      Expression System

      Escherichia coli

    • Affinity Tag

      His Tag (C-term)

      Storage Buffer

      Lyophilized from a 0.2 µm filtered solution of PBS, pH 8.0.

    • Purity

      >98% as determined by SDS-PAGE analysis.

      Molecular weight

      The protein has a calculated MW of 55.03 kDa.
      The protein migrates as 48-63 kDa under reducing condition (SDS-PAGE analysis).

    • Form

      Lyophilized

  • Background
    • Background

      Zika virus (ZIKV), a member of Flaviviridae family,is an emerging disease that is spread by Aedes mosquitoes. ZIKV is composed of a single polyprotein that is cleaved into three structural proteins: capsid (C), precursor of membrane (prM), envelop (E) and seven non-structural proteins: NS1, NS2A, NS2B, NS3, NS4A, NS4B and NS5. ZIKV E protein is containing 501 amino acids with polyhistidine tag at the C-terminus. ZIKV E protein composes the majority of the virion surface and it is involved with replication, such as host cell binding and membrane fusion.

      Synonyms

      Genome polyprotein

    • Uniprot ID

      #Q32ZE1

      Sequence Note

      Ile291-Ala790

  • Instruction
    • Reconstitution

      It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

      Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    • Stability & Storage

      This product is stable after storage at:

      • -20°C for 12 months in lyophilized state from date of receipt.
      • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

      Avoid repeated freeze/thaw cycles.

  • Image
    SDS-PAGE analysis of recombinant ZIKV E protein.

    SDS-PAGE analysis of recombinant ZIKV E protein.

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  • Datasheet & Documents
    MSDS
    Manual
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